| POST
GRADUATE DEGREE STANDARD |
|
UNIT I
History of Microbiology:-
Concept of origin of life - abiogenesis - biogenesis
- Spontaneous genesation theory. Contribution by Luis
Pasteur, S.A. Wakmann, Alexander Flemming, Robert koch,
Winogradsky, Stanely, Iwenewsky, H.W.Conn, EswinSmith.
UNIT
II
Methods in Microbiology:-
Sterilization - Discirfection; Isotation, Purification
and preservation of Mecrobes, Principles of Staining
of micro organisms, Microscopy: Light Phase contract,
Epifluorescence and Election miscroscopy - Assay of
antibiotics. Preparation of culture media.
UNIT
III
Protists:-
Archaeobacteria, Procasyotic and Eucaryotic microorganisms
and their differentiation. Evolution and classification
of microorganisms, Protozoa, Algae,Fungi bacteria,Actinomycotes,
Rickettside, Mycoplasma and virsuses. Modern approches
to taxonomy.
UNIT
IV
Mosphyology and cycology:-
cycology of microfial cell- comparision of the cytological
features of different groups of microorganisms - chemical
nature of cell wall. protoplasm, nucleus, granular materials
and other inclusions of microbial cells.
UNIT
V
Physiology of Microorganisms:-
Growth and metabolism - growth phases, kinetics and
influence of environmental parameters, nutritional groupings,
metabolism of Carbohydrates, Nitrogen, Lipids, and nucleic
acids, Electron transport, Microbial enzymes and regulation.
UNIT
VI
Principles of immunotechnology:-
Antigen and antibody reactions, hypersenitivity, hybridoma,
monoclonal serological techniques. Host microbe interaction
- virulent factors - pathogenicity - infection - resistance.
UNIT
VII
Advanced Techniques:-
Principles and Application of Gel filtraction, ion exchange
and affinity, high pressure liquid chromatography (HPLC)
as chromatography (GC) Electrophoresis, Electrofocussing,
ultracentrifiogation ELISA technique Fluorescent Antibody
Technique, Radioactive isotopes autoradiography.
UNIT
VIII
Microbial genetics:-
mutations and variations genetics of Neurospora, Aspergillus
and Saceharomyces hetero caryosis parasexuality genetics
of bacteria and bacteriophages, plasmids episomes and
tronsposans Transformation, Transduction Conjugation,
Gentic Improvement of microorganisms, geneclouing and
modern R - DNA- techniques to Improve biotechnologically
important microorganisms.
UNIT
IX
Microbial ecology:-
principles and their application to microbial ecosystems.
Methods of studying Microbial ecosystem. Iterrelationship
of microorganisms - biological equillibrium.
UNIT
X
Statistics:-
elementary principles of statistics, mean, mods and
medion, chisquare, correlation and regressions, analysis
of variance. Statistical methods in biologicalresearch-use
of Duncan's multiplication range test.
PAPER- II
APPLIED
MICROBIOLOGY
UNIT I
Industrial Microbiology:- Production of ethanol and
alcoholic beverages, Organic acids, Polysaccharids,
Aminoacids - Vitamins - enzymes growth regulators, antibiotics
fermentation techniques, continuous cultivation of microorganisms,
patents terms and regulations. Microbial leaching of
ores.
UNIT
II
Agricultural Microbiology:Distribution of microorganisms,
organic matter decomposition - Microbiology and biochemistry.
Biofertilizers Biological Nitrogen fixation, nitrification
denitrification and microbial transofrmation of iron,
sulphur and Phosphorus Ecto and Endo mycorrhizal association
in plants and their significance, Microbial pesiticides
- Microbial degradation of pesticides.
UNIT
III
Biomass conversion:- Production of biomass with respect
to microbial energy conversion-Ethanol from biomass,
Biogas Technology - Use of biomass for methaneogenesis
- Biogas plants. hydrogen fuel from microbes.
UNIT
IV
Plant microbiology:-
Concept of phyllosphere, rhizosphere and spermosphere,
modification of rhizosphere effect, R.S, ratio, root
exudates and their influence on plant growth and microbial
community.
UNIT
V
Environmental microbiology:-Microbiology of water andAir-
Microbial assessment of water quality, safe disposal
of sewage and industrial effluents. Waste water treatement
and pollution control. Management of organic wastes:
Utilization of agricultural wastes throughmicrobial
degradation. Microbial composting, disposal of municipal,
domestic and industrial wastes through microbial processes.
Recycling of sewage water, Microbial deodorization and
decolouration of effluents.
UNIT
VI
Food Microbiology:-Role of microbes in preparation of
sauer- Kraut, bread and pickles. Preservation of food,
sources of spoilage of food, food infection, food toxicity,
and control of food borne microorganisms, food adultration
and legislation. Microbes as food Single cell protein
production, Mushroom production.
UNIT
VII
Dairy Bacteriology:- Microbiology of milk - Pathogenic
bacteria in milk - Spoilage of fresh milk and milk products
- Prevention. Preservation of milk and milk products
- production of fermented Dairy products.
UNIT
VIII
Microbiology of Fibres:Microbial retting of fibres -
Jute - flax - coir, Biodeterioration of cotton - jute,
preventon of damage of fibres.
UNIT
IX
Microbial diseases and their control:-
Plant diseases:-
Damping off, rots and wilts, mildews, smuts and rusts
and leaf spots.
Animal diseases:-
Anthrax foot and mouth disease - Bovine - Rinderpest
Human diseases:- Tuber culosis - leprosy - tetanus -
Diptheria, Typhoid - cholera - HIV's.
UNIT
X
Microbial Biotechnology:-Developments in microbial biotechnology
and Genetic manipulation - recombinant DNA technology
- techniques Applications of biotechnology - production
of antibioties, enzymes, Insulin, growth hormones -
interferons - monoclonal antibodies.
|
