Unit-1-Biomolecules
Corbohydrates-
Classification, Structure- Reactions-Oligosaccharaides
- Poliysaccharaides-Structural Polysaccharides-Lipids-Classification-Simple-compound
-Derived-biomembranes- Amino acids-Classification-reactions-peptides-Primary
structures-Glutathione, Oxytocin -vasopressin-Proteins-Classification-
Properties-Structure-Myoglobin & hemoglobin-Vitamins-Fat
soluble-water soluble-Sources-structure-physiological
functions
Unit-II
Techniques
PH
scale-Buffer solutions, buffer systems of blood,-Chromatography-principle,
materials, methods and applications:- Paper chromatography,
TLC, GLC, Adsorption, ion exchange, affinity chromatography
and molicular sieve: principle of HPLC, Molecular weight
determination by molecular sieve method.
Electrophoresis-Ultracentrifuge-Separation of Cell organelles-Colorimetry-Spectrophotometry-Fluorimetry-Flame
photometer-Tracer-Techniques-Applications of radioisotopes
in biological sciences-Radioisotopes in medical sciences.
Unit-III-
Enzymes
Enzymes-
six main classes- Catalysis-Extraction, purification
and characterization of enzyme kinetics-Enzyme-inhibition-Ribozymes-Isozymes-Coenzymes-Multienzyme
complex. Mechanism of enzyme action-immobilized enzymes.
Unit-IV-
Intermediary Metabolism
Bioenergetics-high
energy compounds-free energy of hydrolysis-Glycolysis-TCA
cycle-Gluconeogneisis-Glycogenesis-Pentose Phosphate
pathway-Electron transport chain-Oxidative phosphorylation-Oxidation
of fattyacids-beta oxidation-alpha oxidation-omega oxidation-Biosynthesis
of-fatty acids and Cholesterol-Catabolism of amino acid-Oxidative
deamination-transmination-decraboxylation-Metabolism
of Purines-Pyrimidines.
Unit-V-
Physiology
Digestive
system-Respiratory system-Blood-Body fluids-Nervous
system-propagation of nerve impulse synaptic transmission-Neurotransmitters-Myosin
and Actin and regulatory proteins-mechanism of contraction-Structure
of nephron-renal regulation-Spermatogenesis-menstrual
cycle-Pregnancy and lactation-steroid and glycoprotein-hormones-second
messenger concept-CAMP.
Unit
- VI - Molecular Biology
Bacterial
transformation-transduction and conjugation-DNA replication
in prokaryotes-eukaryotic replication-Prokaryotic transcription-Initiation,
elongation and termination -Eukaryotic gene-necleosomes-transcription
factors-Processing of mRNA-Reverse transcription-Genetic
code-tRNA-Translation-Protein synthesis in prokaryotes.-Recombination-DNA
repair mechanism-Excision repair-gene regulation-gene
mutation-reversion-Bacterial transposons.
Unit-VII
- Immunology
Antibody
mediated-Cell mediated response-lymphoid organs-neutrophils-macrophages-phagocytosis-Antigens
-antigenic determinants-haptans-Classes of Immunoglobulins-Antigen-antibody
interactions in-vivo-complement component-Immuno electrophoresis-Cytokines.
Unit-VIII-
Clinical Biochemistry
Diabetes
mellitus-blood sugar GTT-Complications of diabetes-mellitus-Plasma
lipids-hyperlipoproteinemia-Type I, II, III, IV, and
V-lipoproteinemia-atherosclerosis-Hypercholesterolemia-tests
of gastric function-Liver function tests-jaundice-Serum
enzymes in liver disease-(SGPT) & SGOT)-Kidney function
tests-creatinine and urea-Clearance.
Unit-IX
- Animal and Plant Cell Biotechnology
Plant
Tissue Culture-Micropropagation-Gene transfer in plants-Ti
Plasmid vectors-Protoplast fusion-Transgenic plants-Pest
resistance-Mammalian cell culture- Cell-lines-Suspension
culture; immobilized-cultivation-Gene transfer into
mammalian cells-vectors; in vitro fertilization (IVF);
embryo cloning.
Unit-X
- Plant Bio-chemistry
Plant
Cell- Cell wall-Organelles-Transport mechanisms-Absorption-Translocation
of Water-Transpiration-Photosynthesis- Pigments-light
obsorption- Photosystem-I-Photosystem-II-Calvin’s
cycle (C3-Plants).- (C4 Plants)-Photorespiration-Nitrogen
cycle-Nitrogen fixation.
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